EasyPro Universal Inositol Triphosphate(IP3)ELISA Kit

Universal

InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

Competitive

10000pg/mL

49.3 pg/mL

156.25-10000 pg/mL

45min

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Bone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IP3 Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for IP3 are added to the micro ELISA plate wells. IP3 in samples (or standards) competes with a fixed amount of IP3 on the solid phase supporter for sites on the HRP linked detection antibody specific to IP3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.