EasyPro Universal Folic Acid(FA)ELISA Kit

Universal

VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate

Competitive

10000pg/mL

14.7 pg/mL

156.25-10000 pg/mL

45min

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Hematology;Reproductive science;Nutrition metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with FA Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for FA are added to the micro ELISA plate wells. FA in samples (or standards) competes with a fixed amount of FA on the solid phase supporter for sites on the HRP linked detection antibody specific to FA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of FA in the samples is then determined by comparing the OD of the samples to the standard curve.