EasyPro Universal 17-Hydroxyprogesterone(17-OHP)ELISA Kit

Universal

17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone

Competitive

20000pg/mL

87.3 pg/mL

312.5-20000 pg/mL

45min

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Endocrinology;Reproductive science;Hormone metabolism;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 17-OHP Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for 17-OHP are added to the micro ELISA plate wells. 17-OHP in samples (or standards) competes with a fixed amount of 17-OHP on the solid phase supporter for sites on the HRP linked detection antibody specific to 17-OHP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve.