EasyPro Series ELISA Kits

EasyPro Rat Thyrotropin Releasing Hormone(TRH)ELISA Kit

Product No

FY-ER1641S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL

Assay Time

45min

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Product Details
Product Name in English:
EasyPro Rat Thyrotropin Releasing Hormone(TRH)ELISA Kit
Species:
Rat
Alternative Name:
Thyrotropin-releasing hormone, TRH, Protirelin, TSH-releasing factor, Thyroliberin, Thyrotropin-releasing factor, TRF
Uniport ID:
P01150
Assay Type:
Competitive
Standard:
500pg/mL
Sensitivity:
4.69 pg/mL
Detection Range:
7.82-500pg/mL
Assay Time:
45min
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL
Research:
Tags & Cell Markers;Cell Biology;Cancer
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TRH Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for TRH are added to the micro ELISA plate wells. TRH in samples (or standards) competes with a fixed amount of TRH on the solid phase supporter for sites on the HRP linked detection antibody specific to TRH. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of TRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
500 0.182 0.186 0.184
250 0.32 0.326 0.323
125 0.539 0.547 0.543
62.5 0.87 0.885 0.878
31.25 1.291 1.303 1.297
15.63 1.748 1.77 1.759
7.82 2.057 2.088 2.073
0 2.537 2.562 2.55
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 87-99 93
EDTA plasma (n=8) 80-95 87
Cell culture media (n=8) 92-106 100
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 95-102 88-97 93-107
Average (%) 102 89 98
1:4 Range (%) 83-96 88-97 85-94
Average (%) 87 95 93
1:8 Range (%) 97-105 96-105 87-96
Average (%) 100 103 94
1:16 Range (%) 85-97 81-96 92-101
Average (%) 88 88 94
Assay Procedures
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