EasyPro Series ELISA Kits

EasyPro Mouse Orphanin FQ/Nociceptin(OFQ/N)ELISA Kit

Product No

FY-EM1756S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL

Assay Time

45min

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Product Details
Product Name in English:
EasyPro Mouse Orphanin FQ/Nociceptin(OFQ/N)ELISA Kit
Species:
Mouse
Alternative Name:
PPNOC, nocistatin|propronociceptin
Assay Type:
Competitive
Standard:
500pg/mL
Sensitivity:
4.69 pg/mL
Detection Range:
7.82-500pg/mL
Assay Time:
45min
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50μL
Research:
Metabolic pathway;
Test Principle:
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with OFQ/N Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for OFQ/N are added to the micro ELISA plate wells. OFQ/N in samples (or standards) competes with a fixed amount of OFQ/N on the solid phase supporter for sites on the HRP linked detection antibody specific to OFQ/N. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of OFQ/N in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
500 0.188 0.19 0.189
250 0.326 0.329 0.328
125 0.545 0.547 0.546
62.5 0.876 0.886 0.881
31.25 1.297 1.298 1.298
15.63 1.754 1.762 1.758
7.82 2.063 2.091 2.077
0 2.543 2.558 2.551
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 90-105 97
EDTA plasma (n=8) 80-95 87
Cell culture media (n=8) 88-102 95
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 89-102 93-102 81-94
Average (%) 97 101 92
1:4 Range (%) 82-94 80-93 96-105
Average (%) 93 83 98
1:8 Range (%) 85-96 91-100 92-103
Average (%) 88 96 99
1:16 Range (%) 80-90 88-95 82-96
Average (%) 87 95 95
Assay Procedures
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