EasyPro Human Alpha Crosslaps(α-CTx)ELISA Kit

Human

a-CTXI, aCTX-I, A-Cr, Type I Collagen C-Telopeptide-Related Fraction

P50983

Competitive

6000pg/mL

56.25 pg/mL

93.75-6000pg/mL

45min

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Tumor immunity;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with α-CTx Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for α-CTx are added to the micro ELISA plate wells. α-CTx in samples (or standards) competes with a fixed amount of α-CTx on the solid phase supporter for sites on the HRP linked detection antibody specific to α-CTx. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of α-CTx in the samples is then determined by comparing the OD of the samples to the standard curve.