Product No
FY-ER3452SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
100 | 2.342 | 2.372 | 2.357 | 2.326 |
50 | 1.54 | 1.554 | 1.547 | 1.516 |
25 | 0.937 | 0.946 | 0.942 | 0.911 |
12.5 | 0.505 | 0.513 | 0.509 | 0.478 |
6.25 | 0.25 | 0.252 | 0.251 | 0.22 |
3.13 | 0.135 | 0.136 | 0.136 | 0.105 |
1.57 | 0.074 | 0.075 | 0.075 | 0.044 |
0 | 0.031 | 0.031 | 0.031 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 87-95 | 91 |
EDTA plasma (n=8) | 96-107 | 102 |
Cell culture media (n=8) | 80-96 | 87 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 89-97 | 80-92 | 95-104 |
Average (%) | 91 | 83 | 102 | |
1:4 | Range (%) | 85-94 | 86-102 | 82-96 |
Average (%) | 89 | 91 | 95 | |
1:8 | Range (%) | 80-96 | 87-103 | 92-103 |
Average (%) | 81 | 101 | 95 | |
1:16 | Range (%) | 85-99 | 85-101 | 90-99 |
Average (%) | 93 | 91 | 95 |