Product No
FY-ER3394SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
5000 | 2.501 | 2.546 | 2.524 | 2.458 |
2500 | 1.645 | 1.675 | 1.66 | 1.594 |
1250 | 0.979 | 0.983 | 0.981 | 0.915 |
625 | 0.46 | 0.467 | 0.464 | 0.398 |
312.5 | 0.251 | 0.251 | 0.251 | 0.185 |
156.25 | 0.153 | 0.155 | 0.154 | 0.088 |
78.13 | 0.101 | 0.102 | 0.102 | 0.036 |
0 | 0.066 | 0.066 | 0.066 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 90-105 | 97 |
EDTA plasma (n=8) | 82-94 | 88 |
Cell culture media (n=8) | 80-96 | 87 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 80-91 | 85-92 | 88-102 |
Average (%) | 80 | 91 | 91 | |
1:4 | Range (%) | 87-96 | 90-96 | 93-105 |
Average (%) | 95 | 90 | 104 | |
1:8 | Range (%) | 95-103 | 95-102 | 91-99 |
Average (%) | 102 | 99 | 94 | |
1:16 | Range (%) | 85-91 | 87-98 | 83-96 |
Average (%) | 88 | 91 | 89 |