Product No
FY-EM1263SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
500 | 2.478 | 2.513 | 2.496 | 2.453 |
250 | 1.622 | 1.65 | 1.636 | 1.593 |
125 | 0.956 | 0.965 | 0.961 | 0.918 |
62.5 | 0.437 | 0.438 | 0.438 | 0.395 |
31.25 | 0.228 | 0.23 | 0.229 | 0.186 |
15.63 | 0.13 | 0.13 | 0.13 | 0.087 |
7.82 | 0.078 | 0.079 | 0.079 | 0.036 |
0 | 0.043 | 0.043 | 0.043 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 82-95 | 88 |
EDTA plasma (n=8) | 83-98 | 91 |
Cell culture media (n=8) | 91-99 | 95 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 85-96 | 86-101 | 92-105 |
Average (%) | 92 | 100 | 93 | |
1:4 | Range (%) | 87-101 | 86-101 | 89-102 |
Average (%) | 98 | 86 | 98 | |
1:8 | Range (%) | 89-102 | 86-101 | 92-101 |
Average (%) | 98 | 93 | 94 | |
1:16 | Range (%) | 85-98 | 87-96 | 88-102 |
Average (%) | 97 | 91 | 95 |