Product No
FY-EM3227SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
6000 | 2.761 | 2.783 | 2.772 | 2.742 |
3000 | 1.699 | 1.722 | 1.711 | 1.681 |
1500 | 0.962 | 0.974 | 0.968 | 0.938 |
750 | 0.433 | 0.434 | 0.434 | 0.404 |
375 | 0.215 | 0.215 | 0.215 | 0.185 |
187.5 | 0.108 | 0.11 | 0.109 | 0.079 |
93.75 | 0.0629 | 0.063 | 0.063 | 0.033 |
0 | 0.03 | 0.03 | 0.03 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 82-96 | 87 |
EDTA plasma (n=8) | 86-97 | 92 |
Cell culture media (n=8) | 84-103 | 96 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 88-97 | 85-104 | 82-90 |
Average (%) | 96 | 88 | 85 | |
1:4 | Range (%) | 91-98 | 85-104 | 85-102 |
Average (%) | 91 | 89 | 97 | |
1:8 | Range (%) | 86-103 | 87-106 | 80-92 |
Average (%) | 93 | 94 | 87 | |
1:16 | Range (%) | 80-91 | 83-92 | 87-103 |
Average (%) | 81 | 84 | 92 |