Product No
FY-EM3228SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
20 | 2.353 | 2.373 | 2.363 | 2.293 |
10 | 1.559 | 1.561 | 1.56 | 1.49 |
5 | 0.928 | 0.933 | 0.931 | 0.861 |
2.5 | 0.445 | 0.451 | 0.448 | 0.378 |
1.25 | 0.266 | 0.267 | 0.267 | 0.197 |
0.63 | 0.166 | 0.168 | 0.167 | 0.097 |
0.32 | 0.119 | 0.121 | 0.12 | 0.05 |
0 | 0.069 | 0.07 | 0.07 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 92-105 | 98 |
EDTA plasma (n=8) | 83-95 | 89 |
Cell culture media (n=8) | 87-99 | 93 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 86-97 | 95-104 | 90-99 |
Average (%) | 93 | 99 | 94 | |
1:4 | Range (%) | 93-102 | 95-104 | 90-99 |
Average (%) | 94 | 99 | 95 | |
1:8 | Range (%) | 87-98 | 82-96 | 85-96 |
Average (%) | 96 | 84 | 92 | |
1:16 | Range (%) | 83-97 | 92-103 | 87-98 |
Average (%) | 86 | 102 | 88 |