Product No
FY-EM1587SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
10 | 2.414 | 2.456 | 2.435 | 2.379 |
5 | 1.729 | 1.739 | 1.734 | 1.678 |
2.5 | 1.013 | 1.018 | 1.016 | 0.96 |
1.25 | 0.506 | 0.508 | 0.507 | 0.451 |
0.63 | 0.267 | 0.27 | 0.269 | 0.213 |
0.32 | 0.144 | 0.145 | 0.145 | 0.089 |
0.16 | 0.097 | 0.098 | 0.098 | 0.042 |
0 | 0.056 | 0.056 | 0.056 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 80-95 | 87 |
EDTA plasma (n=8) | 92-105 | 98 |
Cell culture media (n=8) | 95-105 | 100 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 87-96 | 92-101 | 85-93 |
Average (%) | 92 | 100 | 91 | |
1:4 | Range (%) | 85-97 | 92-101 | 86-96 |
Average (%) | 94 | 95 | 92 | |
1:8 | Range (%) | 85-94 | 82-98 | 93-104 |
Average (%) | 93 | 94 | 99 | |
1:16 | Range (%) | 87-96 | 89-97 | 89-97 |
Average (%) | 90 | 90 | 95 |