Product No
FY-EM2082SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
40 | 2.364 | 2.365 | 2.365 | 2.335 |
20 | 1.524 | 1.553 | 1.539 | 1.509 |
10 | 0.85 | 0.853 | 0.852 | 0.822 |
5 | 0.45 | 0.456 | 0.453 | 0.423 |
2.5 | 0.23 | 0.234 | 0.232 | 0.202 |
1.25 | 0.127 | 0.128 | 0.128 | 0.098 |
0.63 | 0.08 | 0.081 | 0.081 | 0.051 |
0 | 0.03 | 0.03 | 0.03 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 80-92 | 85 |
EDTA plasma (n=8) | 92-105 | 98 |
Cell culture media (n=8) | 85-97 | 91 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 85-105 | 86-94 | 85-95 |
Average (%) | 102 | 92 | 87 | |
1:4 | Range (%) | 87-98 | 86-94 | 89-97 |
Average (%) | 94 | 90 | 94 | |
1:8 | Range (%) | 91-102 | 85-95 | 85-94 |
Average (%) | 94 | 86 | 91 | |
1:16 | Range (%) | 86-102 | 89-99 | 80-96 |
Average (%) | 92 | 96 | 80 |