Product No
FY-EM2427SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
4000 | 2.389 | 2.411 | 2.4 | 2.345 |
2000 | 1.549 | 1.575 | 1.562 | 1.507 |
1000 | 0.875 | 0.876 | 0.876 | 0.821 |
500 | 0.475 | 0.481 | 0.478 | 0.423 |
250 | 0.255 | 0.257 | 0.256 | 0.201 |
125 | 0.152 | 0.153 | 0.153 | 0.098 |
62.5 | 0.105 | 0.107 | 0.106 | 0.051 |
0 | 0.055 | 0.055 | 0.055 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 82-95 | 88 |
EDTA plasma (n=8) | 94-107 | 100 |
Cell culture media (n=8) | 87-95 | 91 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 85-92 | 87-98 | 86-92 |
Average (%) | 89 | 94 | 92 | |
1:4 | Range (%) | 87-97 | 87-98 | 89-97 |
Average (%) | 93 | 93 | 92 | |
1:8 | Range (%) | 83-96 | 88-97 | 87-98 |
Average (%) | 94 | 95 | 89 | |
1:16 | Range (%) | 95-102 | 91-103 | 92-105 |
Average (%) | 98 | 97 | 99 |