EasyPro Series ELISA Kits

EasyPro Mouse Hepcidin(Hepc)ELISA Kit

Product No

FY-EM2736S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL

Assay Time

1.5H

Price($)

Size

Purchase details

Contact supplier to negotiate price details

100% Quality Assurance

Fast Shipping

Product Details
Product Name in English:
EasyPro Mouse Hepcidin(Hepc)ELISA Kit
Species:
Mouse
Alternative Name:
Hepcidin Antimicrobial Peptide
Uniport ID:
Q9EQ21
Assay Type:
Sandwich
Standard:
8000pg/mL
Sensitivity:
75 pg/mL
Detection Range:
125-8000pg/mL
Assay Time:
1.5H
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL
Research:
Enzyme & Kinase;Infection immunity;Hepatology;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Hepc. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for Hepc are added to the micro ELISA plate wells. Hepc in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Hepc. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Hepc. You can calculate the concentration of Hepc in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
8000 2.47 2.513 2.492 2.439
4000 1.619 1.639 1.629 1.576
2000 0.977 0.996 0.987 0.934
1000 0.469 0.473 0.471 0.418
500 0.242 0.243 0.243 0.19
250 0.132 0.134 0.133 0.08
125 0.087 0.089 0.088 0.035
0 0.052 0.053 0.053 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 80-92 85
EDTA plasma (n=8) 82-95 88
Cell culture media (n=8) 94-107 100
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 91-102 86-102 98-105
Average (%) 101 86 99
1:4 Range (%) 86-102 87-96 92-101
Average (%) 94 95 96
1:8 Range (%) 87-99 89-98 85-105
Average (%) 94 95 96
1:16 Range (%) 99-105 83-103 87-98
Average (%) 102 93 91
Assay Procedures
Related literature
Related Products