EasyPro Series ELISA Kits

EasyPro Mouse Immunoglobulin A(IgA)ELISA Kit

Product No

FY-EM2792S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL

Assay Time

1.5H

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Product Details
Product Name in English:
EasyPro Mouse Immunoglobulin A(IgA)ELISA Kit
Species:
Mouse
Alternative Name:
IgA
Assay Type:
Sandwich
Standard:
400ng/mL
Sensitivity:
3.75 ng/mL
Detection Range:
6.25-400ng/mL
Assay Time:
1.5H
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL
Research:
Cell Biology; Cancer; Metabolism
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IgA. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for IgA are added to the micro ELISA plate wells. IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to IgA. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IgA. You can calculate the concentration of IgA in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
400 2.401 2.406 2.404 2.336
200 1.561 1.578 1.57 1.502
100 0.887 0.9 0.894 0.826
50 0.487 0.489 0.488 0.42
25 0.267 0.27 0.269 0.201
12.5 0.164 0.164 0.164 0.096
6.25 0.117 0.118 0.118 0.05
0 0.067 0.068 0.068 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 85-97 91
EDTA plasma (n=8) 81-95 88
Cell culture media (n=8) 83-95 89
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 95-106 95-102 88-97
Average (%) 97 98 92
1:4 Range (%) 87-101 98-106 86-97
Average (%) 92 103 90
1:8 Range (%) 82-97 87-98 85-103
Average (%) 96 94 101
1:16 Range (%) 87-102 91-102 89-97
Average (%) 89 94 96
Assay Procedures
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