Product No
FY-EM1546SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
200 | 2.35 | 2.36 | 2.355 | 2.288 |
100 | 1.556 | 1.577 | 1.567 | 1.5 |
50 | 0.925 | 0.935 | 0.93 | 0.863 |
25 | 0.442 | 0.449 | 0.446 | 0.379 |
12.5 | 0.263 | 0.268 | 0.266 | 0.199 |
6.25 | 0.163 | 0.166 | 0.165 | 0.098 |
3.13 | 0.116 | 0.118 | 0.117 | 0.05 |
0 | 0.066 | 0.067 | 0.067 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 85-97 | 91 |
EDTA plasma (n=8) | 97-105 | 101 |
Cell culture media (n=8) | 83-99 | 95 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 85-102 | 80-97 | 85-94 |
Average (%) | 87 | 96 | 94 | |
1:4 | Range (%) | 80-92 | 96-104 | 87-103 |
Average (%) | 91 | 97 | 88 | |
1:8 | Range (%) | 87-98 | 85-93 | 97-105 |
Average (%) | 92 | 93 | 101 | |
1:16 | Range (%) | 95-102 | 89-97 | 80-90 |
Average (%) | 98 | 95 | 80 |