Product No
FY-EM1040SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
1000 | 2.476 | 2.525 | 2.501 | 2.454 |
500 | 1.596 | 1.623 | 1.61 | 1.563 |
250 | 0.903 | 0.915 | 0.909 | 0.862 |
125 | 0.468 | 0.474 | 0.471 | 0.424 |
62.5 | 0.259 | 0.264 | 0.262 | 0.215 |
31.25 | 0.153 | 0.156 | 0.155 | 0.108 |
15.63 | 0.102 | 0.102 | 0.102 | 0.055 |
0 | 0.046 | 0.047 | 0.047 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 89-103 | 96 |
EDTA plasma (n=8) | 80-93 | 85 |
Cell culture media (n=8) | 82-95 | 88 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 88-102 | 81-94 | 82-96 |
Average (%) | 101 | 93 | 91 | |
1:4 | Range (%) | 86-97 | 81-94 | 80-92 |
Average (%) | 88 | 84 | 81 | |
1:8 | Range (%) | 89-97 | 96-105 | 83-96 |
Average (%) | 95 | 96 | 94 | |
1:16 | Range (%) | 80-95 | 92-103 | 95-107 |
Average (%) | 95 | 95 | 101 |