Product No
FY-EM1574SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
2000 | 2.319 | 2.354 | 2.337 | 2.302 |
1000 | 1.525 | 1.542 | 1.534 | 1.499 |
500 | 0.894 | 0.91 | 0.902 | 0.867 |
250 | 0.411 | 0.413 | 0.412 | 0.377 |
125 | 0.232 | 0.236 | 0.234 | 0.199 |
62.5 | 0.132 | 0.132 | 0.132 | 0.097 |
31.25 | 0.085 | 0.085 | 0.085 | 0.05 |
0 | 0.035 | 0.035 | 0.035 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 82-95 | 88 |
EDTA plasma (n=8) | 88-102 | 95 |
Cell culture media (n=8) | 95-107 | 101 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 80-92 | 80-92 | 92-104 |
Average (%) | 81 | 80 | 93 | |
1:4 | Range (%) | 80-93 | 86-102 | 82-96 |
Average (%) | 84 | 96 | 84 | |
1:8 | Range (%) | 94-106 | 87-103 | 95-102 |
Average (%) | 102 | 88 | 99 | |
1:16 | Range (%) | 83-97 | 85-101 | 87-98 |
Average (%) | 95 | 95 | 89 |