Product No
FY-EM3077SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
100 | 2.362 | 2.408 | 2.385 | 2.334 |
50 | 1.56 | 1.58 | 1.57 | 1.519 |
25 | 0.957 | 0.962 | 0.96 | 0.909 |
12.5 | 0.525 | 0.529 | 0.527 | 0.476 |
6.25 | 0.27 | 0.271 | 0.271 | 0.22 |
3.13 | 0.155 | 0.157 | 0.156 | 0.105 |
1.57 | 0.094 | 0.096 | 0.095 | 0.044 |
0 | 0.051 | 0.051 | 0.051 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 80-95 | 86 |
EDTA plasma (n=8) | 80-97 | 88 |
Cell culture media (n=8) | 82-94 | 88 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 86-98 | 87-91 | 89-97 |
Average (%) | 89 | 88 | 93 | |
1:4 | Range (%) | 91-103 | 85-93 | 85-94 |
Average (%) | 102 | 90 | 94 | |
1:8 | Range (%) | 85-97 | 89-97 | 87-96 |
Average (%) | 92 | 91 | 95 | |
1:16 | Range (%) | 87-96 | 85-93 | 85-97 |
Average (%) | 93 | 88 | 92 |