EasyPro Series ELISA Kits

EasyPro Mouse Pulmonary surfactant-associated protein A1(SP-A1)ELISA Kit

Product No

FY-EM3035S

Sample Type

Serum, Tissue homogenate, Cell lysate, Cell culture supernatant, Alveolar lavage fluid;Sample Volume=50uL

Assay Time

1.5H

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Product Details
Product Name in English:
EasyPro Mouse Pulmonary surfactant-associated protein A1(SP-A1)ELISA Kit
Species:
Mouse
Alternative Name:
MGC133365, PSAP, PSPA, SFTP1, SFTPA1
Uniport ID:
P35242
Assay Type:
Sandwich
Standard:
20ng/mL
Sensitivity:
0.19 ng/mL
Detection Range:
0.32-20ng/mL
Assay Time:
1.5H
Sample Type:
Serum, Tissue homogenate, Cell lysate, Cell culture supernatant, Alveolar lavage fluid;Sample Volume=50uL
Research:
bacterial infection
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SP-A1. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for SP-A1 are added to the micro ELISA plate wells. SP-A1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to SP-A1. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SP-A1. You can calculate the concentration of SP-A1 in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
20 2.32 2.336 2.328 2.292
10 1.526 1.531 1.529 1.493
5 0.895 0.901 0.898 0.862
2.5 0.412 0.414 0.413 0.377
1.25 0.233 0.233 0.233 0.197
0.63 0.133 0.135 0.134 0.098
0.32 0.086 0.086 0.086 0.05
0 0.036 0.036 0.036 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 83-97 91
EDTA plasma (n=8) 82-94 88
Cell culture media (n=8) 94-105 98
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 82-103 88-102 93-102
Average (%) 90 98 101
1:4 Range (%) 81-91 86-97 81-96
Average (%) 85 87 90
1:8 Range (%) 85-101 89-97 93-106
Average (%) 94 96 97
1:16 Range (%) 81-90 80-95 82-95
Average (%) 87 87 82
Assay Procedures
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