Product No
FY-EM2251SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
20 | 2.34 | 2.349 | 2.345 | 2.289 |
10 | 1.546 | 1.558 | 1.552 | 1.496 |
5 | 0.915 | 0.921 | 0.918 | 0.862 |
2.5 | 0.432 | 0.433 | 0.433 | 0.377 |
1.25 | 0.253 | 0.257 | 0.255 | 0.199 |
0.63 | 0.153 | 0.154 | 0.154 | 0.098 |
0.32 | 0.106 | 0.108 | 0.107 | 0.051 |
0 | 0.056 | 0.056 | 0.056 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 95-107 | 101 |
EDTA plasma (n=8) | 80-95 | 86 |
Cell culture media (n=8) | 82-95 | 88 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 80-94 | 88-102 | 81-94 |
Average (%) | 84 | 97 | 89 | |
1:4 | Range (%) | 90-99 | 96-107 | 80-90 |
Average (%) | 92 | 98 | 81 | |
1:8 | Range (%) | 87-103 | 89-103 | 83-95 |
Average (%) | 89 | 89 | 92 | |
1:16 | Range (%) | 83-96 | 80-95 | 86-97 |
Average (%) | 92 | 91 | 87 |