Product No
FY-EH1529SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
4000 | 2.37 | 2.38 | 2.375 | 2.339 |
2000 | 1.53 | 1.554 | 1.542 | 1.506 |
1000 | 0.856 | 0.858 | 0.857 | 0.821 |
500 | 0.456 | 0.46 | 0.458 | 0.422 |
250 | 0.236 | 0.237 | 0.237 | 0.201 |
125 | 0.133 | 0.134 | 0.134 | 0.098 |
62.5 | 0.086 | 0.086 | 0.086 | 0.05 |
0 | 0.036 | 0.036 | 0.036 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 82-95 | 88 |
EDTA plasma (n=8) | 88-99 | 93 |
Cell culture media (n=8) | 80-97 | 88 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 82-94 | 80-92 | 87-99 |
Average (%) | 89 | 91 | 99 | |
1:4 | Range (%) | 85-96 | 86-102 | 85-96 |
Average (%) | 90 | 87 | 85 | |
1:8 | Range (%) | 80-90 | 87-103 | 87-101 |
Average (%) | 86 | 99 | 97 | |
1:16 | Range (%) | 91-99 | 85-101 | 89-102 |
Average (%) | 95 | 99 | 97 |