Product No
FY-EH1546SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
800 | 2.446 | 2.462 | 2.454 | 2.426 |
400 | 1.595 | 1.622 | 1.609 | 1.581 |
200 | 0.953 | 0.965 | 0.959 | 0.931 |
100 | 0.445 | 0.445 | 0.445 | 0.417 |
50 | 0.218 | 0.221 | 0.22 | 0.192 |
25 | 0.108 | 0.109 | 0.109 | 0.081 |
12.5 | 0.063 | 0.064 | 0.064 | 0.036 |
0 | 0.028 | 0.028 | 0.028 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 85-97 | 91 |
EDTA plasma (n=8) | 80-94 | 87 |
Cell culture media (n=8) | 91-105 | 98 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 85-101 | 88-97 | 89-97 |
Average (%) | 99 | 92 | 90 | |
1:4 | Range (%) | 80-96 | 88-97 | 88-104 |
Average (%) | 92 | 88 | 95 | |
1:8 | Range (%) | 80-98 | 91-103 | 87-96 |
Average (%) | 89 | 97 | 92 | |
1:16 | Range (%) | 93-102 | 86-92 | 85-93 |
Average (%) | 100 | 92 | 92 |