EasyPro Series ELISA Kits

EasyPro Human Defensin Beta 1(DEFB1)ELISA Kit

Product No

FY-EH1558S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL

Assay Time

1.5H

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Product Details
Product Name in English:
EasyPro Human Defensin Beta 1(DEFB1)ELISA Kit
Species:
Human
Alternative Name:
DEFβ1, BD1, DEFB-1, DEFB11
Uniport ID:
P60022
Assay Type:
Sandwich
Standard:
2000pg/mL
Sensitivity:
18.75 pg/mL
Detection Range:
31.25-2000pg/mL
Assay Time:
1.5H
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uL
Research:
Signal transduction;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DEFB1. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for DEFB1 are added to the micro ELISA plate wells. DEFB1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to DEFB1. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of DEFB1. You can calculate the concentration of DEFB1 in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
2000 2.331 2.343 2.337 2.289
1000 1.537 1.538 1.538 1.49
500 0.906 0.916 0.911 0.863
250 0.423 0.424 0.424 0.376
125 0.244 0.244 0.244 0.196
62.5 0.144 0.146 0.145 0.097
31.25 0.097 0.099 0.098 0.05
0 0.047 0.048 0.048 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 95-107 101
EDTA plasma (n=8) 82-95 88
Cell culture media (n=8) 85-97 91
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 86-105 87-93 99-104
Average (%) 104 90 102
1:4 Range (%) 84-98 87-93 84-99
Average (%) 93 88 89
1:8 Range (%) 95-102 91-103 81-95
Average (%) 101 96 86
1:16 Range (%) 88-106 82-99 98-107
Average (%) 91 93 100
Assay Procedures
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