EasyPro Human Sialic Acid Binding Immunoglobulin Like Lectin 10(SIGLEC10)ELISA Kit

Human

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Q96LC7

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Enzyme & Kinase;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SIGLEC10. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for SIGLEC10 are added to the micro ELISA plate wells. SIGLEC10 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to SIGLEC10. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SIGLEC10. You can calculate the concentration of SIGLEC10 in the samples by comparing the OD of the samples to the standard curve.