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EasyPro Human Platelet Derived Growth Factor Receptor Alpha(PDGFRα)ELISA Kit

Product No

FY-EH2298S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Assay Time

1.5H

Price($)

300 420

Size

48T 96T

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DataSheet DataSheet 说明书 说明书 MSDS MSDS
Product Details
Product Name in English:
EasyPro Human Platelet Derived Growth Factor Receptor Alpha(PDGFRα)ELISA Kit
Species:
Human
Alternative Name:
PDGFRA,platelet-derived growth factor receptor A,platelet-derived growth factor receptor α,PDGF-R-alpha,PDGFR-alpha,Alpha platelet-derived growth factor receptor,Alpha-type platelet-derived growth factor receptor,CD14 antigen-like family member A,CD14a antigen,Platelet-derived growth factor alpha receptor,Platelet-derived growth factor receptor 2,PDGFR-2,CD_antigen: CD14a,PDGFRA,PDGFR2, RHEPDGFRA
Uniport ID:
P16234
Assay Type:
Sandwich
Standard:
10ng/mL
Sensitivity:
0.1 ng/mL
Detection Range:
0.16-10ng/mL
Assay Time:
1.5H
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL
Research:
Cytokine;Tumor immunity;Infection immunity;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDGFRα. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for PDGFRα are added to the micro ELISA plate wells. PDGFRα in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to PDGFRα. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PDGFRα. You can calculate the concentration of PDGFRα in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
10 2.406 2.417 2.412 2.364
5 1.721 1.746 1.734 1.686
2.5 1.005 1.023 1.014 0.966
1.25 0.498 0.506 0.502 0.454
0.63 0.259 0.26 0.26 0.212
0.32 0.136 0.138 0.137 0.089
0.16 0.089 0.089 0.089 0.041
0 0.048 0.048 0.048 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 83-95 89
EDTA plasma (n=8) 90-105 97
Cell culture media (n=8) 80-95 87
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 93-102 87-98 91-99
Average (%) 93 94 98
1:4 Range (%) 81-96 92-104 83-89
Average (%) 92 104 84
1:8 Range (%) 93-106 82-96 88-104
Average (%) 103 94 104
1:16 Range (%) 82-95 95-102 97-103
Average (%) 83 97 102
Assay Procedures
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