Product No
FY-EH2386SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
4000 | 2.363 | 2.375 | 2.369 | 2.34 |
2000 | 1.523 | 1.529 | 1.526 | 1.497 |
1000 | 0.849 | 0.855 | 0.852 | 0.823 |
500 | 0.449 | 0.449 | 0.449 | 0.42 |
250 | 0.229 | 0.232 | 0.231 | 0.202 |
125 | 0.126 | 0.127 | 0.127 | 0.098 |
62.5 | 0.079 | 0.08 | 0.08 | 0.051 |
0 | 0.029 | 0.029 | 0.029 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 82-98 | 90 |
EDTA plasma (n=8) | 84-98 | 91 |
Cell culture media (n=8) | 80-96 | 88 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 85-96 | 83-103 | 92-106 |
Average (%) | 89 | 88 | 105 | |
1:4 | Range (%) | 87-101 | 82-93 | 92-101 |
Average (%) | 90 | 85 | 95 | |
1:8 | Range (%) | 89-102 | 86-95 | 80-95 |
Average (%) | 93 | 92 | 95 | |
1:16 | Range (%) | 85-98 | 81-96 | 87-96 |
Average (%) | 87 | 85 | 88 |