Product No
FY-EH2427SSample Type
Serum, Plasma, Tissue homogenate and Other biological samples;Sample Volume=50uLAssay Time
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Concentration | OD1 | OD2 | Average OD | Corrected |
---|---|---|---|---|
10 | 2.397 | 2.441 | 2.419 | 2.379 |
5 | 1.712 | 1.731 | 1.722 | 1.682 |
2.5 | 0.996 | 1.004 | 1 | 0.96 |
1.25 | 0.489 | 0.495 | 0.492 | 0.452 |
0.63 | 0.25 | 0.252 | 0.251 | 0.211 |
0.32 | 0.127 | 0.128 | 0.128 | 0.088 |
0.16 | 0.08 | 0.081 | 0.081 | 0.041 |
0 | 0.039 | 0.04 | 0.04 | 0 |
Item | Intra-assay Precision | Intra-assay Precision |
---|---|---|
Sample | 3 | 3 |
Replicate | 9 | 18 |
CV(%) | 5 | 8 |
Sample Type | Range(%) | Average Recovery(%) |
---|---|---|
Serum (n=8) | 88-102 | 95 |
EDTA plasma (n=8) | 80-92 | 86 |
Cell culture media (n=8) | 90-105 | 97 |
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 87-91 | 89-97 | 85-94 |
Average (%) | 89 | 91 | 90 | |
1:4 | Range (%) | 85-93 | 85-94 | 87-96 |
Average (%) | 92 | 90 | 88 | |
1:8 | Range (%) | 89-97 | 87-96 | 92-101 |
Average (%) | 95 | 93 | 95 | |
1:16 | Range (%) | 85-93 | 85-97 | 82-98 |
Average (%) | 86 | 93 | 92 |