EasyPro Human WNT1 Inducible Signaling Pathway Protein 1(WISP1)ELISA Kit

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EasyPro Series ELISA Kits

Product No

FY-EH2641S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Assay Time

1.5H

Price($)

300 420

Size

48T 96T

Purchase details

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DataSheet

说明书 MSDS

MSDS

Product Details

Product Name in English:

EasyPro Human WNT1 Inducible Signaling Pathway Protein 1(WISP1)ELISA Kit

Species:

Human

Alternative Name:

WISP1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 inducible signaling pathway protein 1, WISP1-OT1, WISP1-UT1

Uniport ID:

O95388

Assay Type:

Sandwich

Standard:

5000pg/mL

Sensitivity:

46.88 pg/mL

Detection Range:

78.13-5000pg/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Research:

Endocrinology;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to WISP1. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for WISP1 are added to the micro ELISA plate wells. WISP1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to WISP1. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of WISP1. You can calculate the concentration of WISP1 in the samples by comparing the OD of the samples to the standard curve.

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
5000	2.492	2.525	2.509	2.452
2500	1.636	1.659	1.648	1.591
1250	0.97	0.985	0.978	0.921
625	0.451	0.458	0.455	0.398
312.5	0.242	0.244	0.243	0.186
156.25	0.144	0.146	0.145	0.088
78.13	0.092	0.093	0.093	0.036
0	0.057	0.057	0.057	0

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	87-95	91
EDTA plasma (n=8)	81-95	88
Cell culture media (n=8)	83-97	90

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	85-94	85-103	87-98
1:2	Average (%)	87	101	91
1:4	Range (%)	90-99	85-103	80-92
1:4	Average (%)	92	87	85
1:8	Range (%)	82-101	83-96	96-105
1:8	Average (%)	82	94	104
1:16	Range (%)	91-102	95-102	86-97
1:16	Average (%)	96	97	92

Assay Procedures

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