EasyPro Human Soluble Receptor Activator of Nuclear factor-kB Ligand(sRANKL)ELISA Kit

Human

TNFSF11, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, TNLG6B, tumor necrosis factor superfamily member 11, TNF superfamily member 11

O14788(140~317aa)

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

1.5H

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Tumor immunity;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sRANKL. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for sRANKL are added to the micro ELISA plate wells. sRANKL in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to sRANKL. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of sRANKL. You can calculate the concentration of sRANKL in the samples by comparing the OD of the samples to the standard curve.