EasyPro Series ELISA Kits

EasyPro Human Hypoxia Inducible Factor 1 Alpha(HIF-1α)ELISA Kit

Product No

FY-EH2787S

Sample Type

Tissue homogenate, Cell lysate;Sample Volume=50uL

Assay Time

1.5H

Price($)

Size

Purchase details

Contact supplier to negotiate price details

100% Quality Assurance

Fast Shipping

Product Details
Product Name in English:
EasyPro Human Hypoxia Inducible Factor 1 Alpha(HIF-1α)ELISA Kit
Species:
Human
Alternative Name:
HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
Uniport ID:
Q16665
Assay Type:
Sandwich
Standard:
4000pg/mL
Sensitivity:
37.5 pg/mL
Detection Range:
62.5-4000pg/mL
Assay Time:
1.5H
Sample Type:
Tissue homogenate, Cell lysate;Sample Volume=50uL
Research:
Metabolic pathway;
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HIF-1α. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for HIF-1α are added to the micro ELISA plate wells. HIF-1α in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to HIF-1α. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HIF-1α. You can calculate the concentration of HIF-1α in the samples by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
4000 2.364 2.401 2.383 2.353
2000 1.524 1.533 1.529 1.499
1000 0.85 0.853 0.852 0.822
500 0.45 0.452 0.451 0.421
250 0.23 0.23 0.23 0.2
125 0.127 0.127 0.127 0.097
62.5 0.08 0.081 0.081 0.051
0 0.03 0.03 0.03 0
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 85-97 91
EDTA plasma (n=8) 90-103 96
Cell culture media (n=8) 87-99 93
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 95-106 83-101 89-97
Average (%) 101 99 96
1:4 Range (%) 83-96 83-101 85-94
Average (%) 91 97 94
1:8 Range (%) 87-98 90-99 95-103
Average (%) 93 99 98
1:16 Range (%) 80-89 87-103 80-97
Average (%) 80 94 84
Assay Procedures
Related literature
Related Products