EasyPro Human Prostaglandin E Receptor 2(EP2)ELISA Kit

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EasyPro Series ELISA Kits

Product No

FY-EH3088S

Sample Type

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Assay Time

1.5H

Price($)

300 420

Size

48T 96T

Purchase details

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DataSheet

说明书 MSDS

MSDS

Product Details

Product Name in English:

EasyPro Human Prostaglandin E Receptor 2(EP2)ELISA Kit

Species:

Human

Alternative Name:

Prostaglandin E2 receptor 2,PTGER2,Prostaglandin E2 receptor,Prostaglandin E2 receptor EP2 subtype,PGE receptor EP2 subtype,PGE2 receptor EP2 subtype,Prostanoid EP2 receptor

Uniport ID:

P43116

Assay Type:

Sandwich

Standard:

4000pg/mL

Sensitivity:

37.5 pg/mL

Detection Range:

62.5-4000pg/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50uL

Research:

Infection immunity;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EP2. Samples (or Standards) and and Horseradish Peroxidase (HRP) linked antibody specific for EP2 are added to the micro ELISA plate wells. EP2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to EP2. Excess conjugate and unbound sample or standard are washed from the plate. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of EP2. You can calculate the concentration of EP2 in the samples by comparing the OD of the samples to the standard curve.

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD	Corrected
4000	2.377	2.387	2.382	2.339
2000	1.537	1.545	1.541	1.498
1000	0.863	0.863	0.863	0.82
500	0.463	0.471	0.467	0.424
250	0.243	0.248	0.246	0.203
125	0.14	0.14	0.14	0.097
62.5	0.093	0.094	0.094	0.051
0	0.043	0.043	0.043	0

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

Rate of recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	80-95	87
EDTA plasma (n=8)	90-107	98
Cell culture media (n=8)	87-99	93

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-103	85-92	88-98
1:2	Average (%)	96	88	97
1:4	Range (%)	80-97	89-96	87-103
1:4	Average (%)	89	90	98
1:8	Range (%)	93-102	90-98	89-97
1:8	Average (%)	97	95	96
1:16	Range (%)	87-103	87-98	85-94
1:16	Average (%)	88	95	87

Assay Procedures

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