Rat sE-selectin(soluble E-selectin) ELISA Kit

Rat

E-selectin,SELE,LYAM2,CD62 antigen-like family member E,Endothelial leukocyte adhesion molecule 1, ELAM-1,Leukocyte-endothelial cell adhesion molecule 2,LECAM2,CD62E,Elam-1

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to sE-selectin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to sE-selectin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sE-selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of sE-selectin in the samples is then determined by comparing the OD of the samples to the standard curve.