Rat PC(Pyruvate Carboxylase) ELISA Kit

Rat

PROC, APC, PC, PROC1, THPH3, THPH4, protein C, inactivator of coagulation factors Va and VIIIa, Vitamin K-dependent protein C, Anticoagulant protein C, ?Autoprothrombin IIA, Blood coagulation factor XIV

Sandwich

50ng/mL

0.47 ng/mL

0.79-50ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;CD & Adhesion molecule;Apoptosis;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PC in the samples is then determined by comparing the OD of the samples to the standard curve.