Rat HNF4a(Hepatocyte Nuclear Factor 4 Alpha) ELISA Kit

Rat

Hepatocyte nuclear factor 4-alpha,HNF4A,HNF-4-alpha,Nuclear receptor subfamily 2 group A member 1,Transcription factor 14,TCF-14,Transcription factor HNF-4,HNF4, NR2A1, TCF14

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Infection immunity;Endocrinology;Cardiovascular biology;Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HNF4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HNF4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HNF4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNF4a in the samples is then determined by comparing the OD of the samples to the standard curve.