Rat HSP-60(Heat Shock Protein 60) ELISA Kit

Rat

6 kDa heat shock protein, mitochondrial, 6 kDa chaperonin, Chaperonin 6, CPN6, HSP-65, Heat shock protein 6, HSP-6, Hsp6, Mitochondrial matrix protein P1, Hspd1, Hsp6

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Infection immunity;Kidney biomarker;Hepatology;Urology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP-60. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HSP-60. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP-60, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP-60 in the samples is then determined by comparing the OD of the samples to the standard curve.