Rat RTN4(Reticulon 4) ELISA Kit

Rat

ASY,Foocen,Human NogoA,My43 protein,Nbla271,Nbla1545,neurite growth inhibitor 22,Neurite outgrowth inhibitor,Neuroendocrine-specific protein,Neuroendocrine-specific protein C homolog,NI22/25,Nogo A,NOGO,Nogo protein,NOGO-A,Nogo-B,Nogo-C,NSP,NSP-CL,Reticulon 4,reticulon 5,Reticulon-4,Reticulon-5,RTN 4,RTN 4A,RTN xL,RTN-x,Rtn4,RTN4-A,RTN4-B1,RTN4-B2,RTN4-C,RTN4

Q9JK11

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RTN4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RTN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RTN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RTN4 in the samples is then determined by comparing the OD of the samples to the standard curve.