Rat GRIK2(Glutamate Receptor, Ionotropic, Kainate 2) ELISA Kit

Rat

GRIK2, EAA4, GLR6, GLUK6, GLUR6, GluK2, MRT6, glutamate ionotropic receptor kainate type subunit 2

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GRIK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GRIK2 in the samples is then determined by comparing the OD of the samples to the standard curve.