Rat NFKB-p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Rat

NFKB1, NF-KB1, NF-Kappa-B, NFKB-p15, NFKB-p5, p15, p5, EBP-1, KBF1

Q63369

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFKB-p105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NFKB-p105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFKB-p105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NFKB-p105 in the samples is then determined by comparing the OD of the samples to the standard curve.