Rat SP-D(Pulmonary surfactant-associated protein D) ELISA Kit

Rat

SFTPD, COLEC7, PSP-D, SFTP4, SP-D, surfactant protein D, Lung surfactant protein D, Collectin-7, Pulmonary surfactant-associated protein D

P35248

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Tumor immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SP-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SP-D in the samples is then determined by comparing the OD of the samples to the standard curve.