Rat GAP(Ras Gtpase Activating Protein) ELISA Kit

Rat

RASA1, CM-AVM, CMAVM, GAP, PKWS, RASA, RASGAP, p12GAP, p12RASGAP, RAS p21 protein activator 1, p12

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Infection immunity;Bone metabolism;Rheumatology;Immunodeficiency;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GAP in the samples is then determined by comparing the OD of the samples to the standard curve.