Rat CX3CR1(Chemokine C-X3-C-Motif Receptor 1) ELISA Kit

Rat

CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, C-X3-C motif chemokine receptor 1,CX3C chemokine receptor 1,fractalkine receptor,G-protein coupled receptor 13,C-X3-C CKR-1,Beta chemokine receptor-like 1,CMK-BRL-1,CMK-BRL1

P35411

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CX3CR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CX3CR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CX3CR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CX3CR1 in the samples is then determined by comparing the OD of the samples to the standard curve.