Rat IGFBP-1(Insulin-like Growth Factor Binding Protein 1) ELISA Kit

Rat

IGFBP1, AFBP, IBP1, IGF-BP25, PP12, placental protein 12

P21743

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Endocrinology;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGFBP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IGFBP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IGFBP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IGFBP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.