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Rat MT(Melatonin) ELISA Kit
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Product No

FY-ER3510

Sample Type

Serum, Brain dialysate

Assay Time

1.5H

Price($)

280 400

Size

48T 96T

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Product Details
Product Name in English:
Rat MT(Melatonin) ELISA Kit
Species:
Rat
Alternative Name:
N-Acetyl-5-Methoxytryptamine
Assay Type:
Competitive
Standard:
1000pg/mL
Sensitivity:
9.38 pg/mL
Detection Range:
15.63-1000pg/mL
Assay Time:
1.5H
Sample Type:
Serum, Brain dialysate
Research:
Kidney diseases
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MT in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical Data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration OD1 OD2 Average OD Corrected
1000 0.264 0.265 0.265
500 0.364 0.368 0.366
250 0.558 0.561 0.56
125 0.819 0.822 0.821
62.5 1.114 1.122 1.118
31.25 1.509 1.511 1.51
15.63 1.823 1.828 1.826
0 2.487 2.509 2.498
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Item Intra-assay Precision Intra-assay Precision
Sample 3 3
Replicate 9 18
CV(%) 5 8
Rate of recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type Range(%) Average Recovery(%)
Serum (n=8) 88-103 95
EDTA plasma (n=8) 92-106 99
Cell culture media (n=8) 90-105 97
Linear
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 88-103 95-106 93-102
Average (%) 90 106 102
1:4 Range (%) 83-94 95-106 90-99
Average (%) 89 96 96
1:8 Range (%) 92-105 88-103 95-106
Average (%) 101 90 100
1:16 Range (%) 89-97 81-98 87-101
Average (%) 97 89 100
Assay Procedures
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