Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit

Rat

F1+2

Sandwich

3000pg/mL

28.13 pg/mL

46.88-3000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve.