Rat NUP85(Nucleoporin 85kDa) ELISA Kit

Rat

NUP85, Nup75, FROUNT, nucleoporin 85, Nuclear pore complex protein Nup85, 85 kDa nucleoporin, Nucleoporin Nup75, Pericentrin-1, PCNT1

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NUP85. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NUP85. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NUP85, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NUP85 in the samples is then determined by comparing the OD of the samples to the standard curve.