Rat TP53(Tumor Protein p53) ELISA Kit

Rat

TP53, BCC7, LFS1, P53, TRP53, tumor protein p53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

P10361

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TP53. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TP53 in the samples is then determined by comparing the OD of the samples to the standard curve.