Product Name in English:
Human GSTμ2/GSTm2(Glutathione S Transferase Mu 2, Muscle) ELISA Kit
Alternative Name:
GSTM2, GST4, GSTM, GSTM2-2, GTHMUS, glutathione S-transferase mu 2 (muscle), glutathione S-transferase mu 2
Detection Range:
0.79-50ng/mL
Sample Type:
Serum, Plasma, Tissue homogenate and Other biological samples
Research:
Apoptosis;Tumor immunity;Infection immunity;
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSTμ2/GSTm2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GSTμ2/GSTm2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSTμ2/GSTm2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GSTμ2/GSTm2 in the samples is then determined by comparing the OD of the samples to the standard curve.