Human VEGF-D(Vascular Endothelial Growth Factor D) ELISA Kit

Human

FIGF, VEGF-D, VEGFD, C-fos induced growth factor

O43915

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VEGF-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGF-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VEGF-D in the samples is then determined by comparing the OD of the samples to the standard curve.